Fig. 4.

Semi-quantitative RT-PCR temperature comparisons for selected O. meridionalis genes. RNA was initially extracted from the leaf tissue of seedlings exposed to either control (27 °C) or 24 h of high temperature (45 °C). (A) PCR products were run on a 2.5% agarose gel and stained with Gel Red (Biotium). Abbreviated gene names are; Rubisco activase large (RcaI) and small (RcaII) isoform genes, phosphoglycerate kinase (pgk), transketolase (tkt), phosphoribulokinase (prk), sedoheptulose-1,7-bisphosphatase (sbp), glycine dehydrogenase (gdc-p), ferredoxin-NADP(H) oxidoreductase (fnr), Heat shock protein 90 (hsp90), chaperone 60 (GroEL), heat shock protein 70 (dnaK), and thiamine biosynthesis protein (thi1). All genes underwent 26 cycles of PCR except RcaI, RcaII, and GroEL that underwent 22 cycles and dnaK which required 30 cycles. (B) Spot intensities of bands were compared and relative gene expression after 24 h at 45 °C (heat/control) was calculated with actin used as an internal standard. Values are mean ±SD, n=3.