Table 5.
QRT-PCR relative expression of experimentally determined or predicted gene targets of selected smRNAs
| smRNA | Target genes | Relative change by QRT-PCRa | Target gene details |
| miR156g | AT1G27360 | 1.27 | Squamosa promoter-binding-like 11 |
| & | AT1G27370 | 0.47 | Squamosa promoter-binding-like 10 |
| miR157d | AT1G53160 | 0.41 | Squamosa promoter-binding-like 4 |
| AT1G69170 | 1.29 | Squamosa promoter-binding-like 6 | |
| AT2G42200 | 0.62 | Squamosa promoter-binding-like 9 | |
| AT3G15270 | 1.64 | Squamosa promoter-binding-like 5 | |
| AT3G57920 | 0.31 | Squamosa promoter-binding-like 15 | |
| AT5G43270 | 0.55 | Squamosa promoter-binding-like 2 | |
| AT5G50570 | 1.16 | Squamosa promoter-binding-like 13A | |
| AT5G50670 | AS ABOVE* | Squamosa promoter-binding like 13B | |
| miR157d | AT2G33810 | 1.85 | Squamosa promoter-binding-like 3 |
| miR158a | AT1G64100 | 3.01 | Pentatricopeptide repeat-containing |
| AT3G03580 | 1.2 | Pentatricopeptide repeat-containing | |
| miR159a | AT2G26950 | 1.99 | Myb domain 104 |
| AT2G26960 | 4.57 | Myb domain 81 | |
| AT2G32460 | 2.56 | Myb domain 101 | |
| AT2G34010 | 0.24 | Unknown | |
| AT3G11440 | 1.72 | Myb domain 65 | |
| AT3G60460 | 4.68 | Duo Pollen 1 (R2R3 Myb domain) | |
| AT4G37770 | 0.85 | 1-Amino-cyclopropane-1-carboxylate synthase 8 | |
| AT5G06100 | 1.31 | Myb domain 33 | |
| AT5G18100 | 1.99 | Copper/Zinc superoxide dismutase 3 | |
| AT5G55020 | ND** | Myb domain 120 | |
| miR172a,b | AT2G28550 | 0.46 | Target of Eat1 1 |
| AT2G39250 | 1.85 | Schnarchzapfen | |
| AT3G54990 | 1.01 | Schlafmutze | |
| AT4G36920 | 1.07 | Apetala 2 | |
| AT5G60120 | 0.32 | Target of Eat1 2 | |
| AT5G67180 | 0.57 | Target of Eat1 3 | |
| miR391a | At1g50990 | 6.49 | Protein kinase |
| miR775 | AT1G53290 | 0.69 | Galactosyltransferase family |
| tasiR289 | At1g15940 | ND** | Unknown |
| (TAS1) | At1g51670 | 0.23 | Unknown, contains domain cysteine proteinases |
| At1g68840 | 2.57 | Regulator ATPase of the vacuolar membrane (RAP2.8) | |
| At2g42090 | 6.15 | Actin 9 | |
| At3g27230 | 0.31 | Unknown | |
| At3g61320 | 0.67 | Chloroplast precursor | |
| At4g05360 | 4.65 | Zinc knuckle (CCHC-type) family | |
| At4g29760 | ND** | Unknown, contains domain cysteine proteinases | |
| At4g29770 | 3.91 | Unknown | |
| At5g18040 | 2.44 | Unknown, contains domain cysteine proteinases | |
| At5g18065 | 5.13 | Unknown, contains domain cysteine proteinases | |
| At5g42670 | 1.09 | Agenet domain-containing | |
| At5g52070 | 1.25 | Agenet domain-containing |
Targets in bold have been verified experimentally.
a
Relative change is the ratio of hypoxia-treated sample to the control. The value is an average of at least two biological replications performed in triplicate. *Primers amplified both these genes. **Not detected (ND).