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2-D electrophoresis profiles from white clover plants grown for 140 d at three sulphate concentrations in the nutrient solution (0, 0.095, and 0.380 mM). (A) SDS-PAGE profiles of nodule protein. (B) Immunodetection of the nitrogenase NifH component and leghaemoglobin following SDS-PAGE and Western blotting of nodule extracts with specific antibodies. MM: molecular markers (kDa).
