. 2009 Nov 5;61(1):297–310. doi: 10.1093/jxb/erp305

Table 1.

Relative expression (fold) of a selection of genes associated with various types of cell processes linked to defence and/or cell death in two lines of PtaRHE1 overexpressors compared with the WT

	2^e–ΔΔCT RLR1-1-1			2^e–ΔΔCT RLR1-5-7
	Mean	SE	t-test (P value)	Mean	SE	t-test (P value)
*PtaRHE1*	828 735.18	300 840.44	≤0.01	209 593.44	57 530.96	≤0.01
1. Defence/elicitor-inducible genes
*PR-1a*	256 701.17	176 302.06	≤0.01	8458.53	6585.74	≤0.01
*PR-1b*	120 967.23	85 436.22	≤0.01	1604.42	689.56	≤0.01
*TIZZ*	61 481.94	33 817.36	≤0.01	532.02	296.37	≤0.01
*PR5*	444.98	377.28	≤0.01	9.03	1.85	≤0.01
*SAR8.2a*	102.64	34.82	≤0.01	5.81	2.72	≤0.01
*PR3*	52.36	24.10	≤0.01	2.80	0.53	≤0.01
*HSR201*	22.92	7.78	≤0.01	0.57	0.10	NS^a
*HMGR2*	20.16	10.37	≤0.01	0.68	0.02	≤0.01^a
*WRKY12*	7.45	5.02	≤0.01	0.97	0.36	NS^a
*EDS1*	9.05	3.52	≤0.01	1.25	0.29	NS^a
*PR4*	5.84	1.47	≤0.01	0.98	0.29	NS^a
*WIPK*	2.43	0.57	≤0.01	0.99	0.03	NS^a
LSD1	1.93	0.35	≤0.01^a	0.84	0.39	NS^a
SAMDC	1.59	0.35	NS^a	0.65	0.10	≤0.01^a
BECLIN1	1.59	0.57	NS^a	1.20	0.57	NS^a
DND1	1.59	0.43	NS^a	1.52	0.77	NS^a
ABAKIN	1.49	0.55	NS^a	0.87	0.20	NS^a
NPR1	1.43	0.24	NS^a	1.31	0.21	NS^a
HSP90	1.17	0.56	NS^a	0.77	0.12	NS^a
SIPK	1.01	0.05	NS^a	0.82	0.14	NS^a
S26-PR6	0.95	0.23	NS^a	2.09	1.33	NS
NtMEK2	0.91	0.07	NS^a	0.68	0.14	≤0.05^a
HSR203J	0.78	0.15	NS^a	0.52	0.08	≤0.01^a
Spermidine synthase	0.67	0.03	NS^a	0.81	0.17	NS^a
WIZZ	0.66	0.22	NS^a	0.88	0.32	NS^a
NOA1	0.53	0.09	≤0.05^a	0.78	0.07	NS^a
Polyamine oxidase	0.54	0.27	NS^a	0.52	0.16	≤0.01^a
PR1c	–	–	–	–	–	–
HSR515	–	–	–	–	–	–
2. Cell death induced by proteasome malfunction-related genes
20S proteasome α subunit 3	1.34	0.26	NS^a	1.38	0.54	NS^a
Ubiquitin protease 6	1.35	0.56	NS^a	1.12	0.13	NS^a
Ubiquitin protease 12	1.03	0.15	NS^a	1.38	0.44	NS^a
BS2	1.17	0.42	NS^a	1.06	0.32	NS^a
NAM-like	0.88	0.42	NS^a	1.14	0.11	NS^a
ClpP	0.61	0.17	NS^a	0.81	0.10	NS^a
3. Both defence and proteasome malfunction cell death-induced genes
*PR2*	21 585.51	18 447.37	≤0.01	1169.14	1116.96	≤0.01
*HIN1*	10.89	5.67	≤0.05	0.46	0.40	NS
NtCP-23	2.01	0.70	NS	1.54	0.57	NS^a
SGT1	2.16	0.71	NS	2.27	0.88	NS
4. Apoptosis-related genes
BI-1	1.71	0.46	NS^a	0.93	0.36	NS^a
DAD1	1.19	0.32	NS^a	1.07	0.48	NS^a
5. Leaf senescence-specific gene
CP1	–	–	–	–	–	–
6. Oxidative stress-related genes
APX	1.10	0.28	NS^a	0.70	0.10	≤0.05^a
SOD	0.69	0.07	NS^a	0.58	0.06	≤0.05^a
NbrbohA	2.32	0.98	NS	0.63	0.36	NS^a

The quantitative expression level of each gene was measured by RT-qPCR and each value is the relative accumulation of each gene transcript compared with that of EF1α. The data presented are the mean value of RNA preparations in three different individual plants of each line.

–, no expression detected; NS, not significant.

Genes indicated in bold underwent a significant change in expression in RLR1-1-1.

A difference in expression lower than 2-fold up or 2-fold down was not considered in this study.