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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Infect Dis. 2010 Jan 1;201(1):160–169. doi: 10.1086/648589

FIGURE 5.

FIGURE 5

Decreased miR-513 expression impacts C. parvum-induced B7-H1 protein expression in cholangiocytes. (A) Transfection of miR-513 precursor reduces C. parvum-induced B7-H1 protein expression. H69 cells were transfected with miR-513 precursor or a control non-specific precursor for 48 h, exposed to C. parvum oocysts for 24 h followed and then assayed for B7-H1 by Western blot. A representative Western blot from three independent experiments and densitometric levels of B7-H1 signals are also shown. (B) MicroRNA-513 precursor transfection does not affect C. parvum-induced B7-H1 mRNA expression. H69 cells were transfected with miR-513 precursor or a control non-specific precursor for 48 h and then exposed to viable C. parvum oocysts for 24 h followed by real-time PCR analysis for B7-H1 mRNA. *, p < 0.05, vs. non-infected control cells.