TST positivity is not associated with alteration in Th1 or Th2 cytokines. PBMC from TST+ (n = 15) and TST− (n = 10–15) patients were stimulated with PPD (10 μg/ml) or Mtb CFA (10 μg/ml) or anti-CD3 (5 μg/ml) for 24 h, and levels of A Th1 cytokines IL-2, IFN-γ, TNF-α, and IL-12 as well as B Th2cytokines IL-4, IL-5, and IL-13 were measured by ELISA. Results are shown as net cytokine production over media control. P values were calculated using the Mann-Whitney test and only statistically significant P values are shown.