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TST positivity is not associated with changes in the expression of TGFβ or IL-10. PBMC from TST+ (n = 10–15) and TST− (n = 10) patients were stimulated with PPD (10 μg/ml), Mtb CFA (10 μg/ml), or anti-CD3 (5 μg/ml) for 24 h, and TGFβ mRNA and IL-10 cytokine levels were measured by real-time RT-PCR and ELISA, respectively. Results are shown as fold change or as net cytokine production over media control. P values were calculated using the Mann-Whitney test.
