. 2009 Dec 24;5(12):e1000708. doi: 10.1371/journal.ppat.1000708

Table 2. Oligonucleotides used in this study.

Primer	Sequence (5′-3′)
NleH1 XhoI-f -FLAG/HA	G₂CTCGAGATGT₂ATCGC₂ATAT₂CTGTA₃T₃G₃
NleH1 BamHI-f-His	G₄ATC₂ATGT₂ATCGC₂ATAT₂CTGTA₃T₃G₃
NleH1 KpnI-r-FLAG	C₂G₂TAC₂A₂T₄ACT₂A₂TAC₂ACACTA₂TA₂GATCT₂GCT₃C₂GC₂
NleH1 XhoI-r-His	C₃TCGAGA₂T₄ACT₂A₂TAC₂ACACTA₂TA₂GATCT₂GCT₃C₂GC₂
NleH2 XhoI-f-FLAG/HA	G₂CTCGAGATGT₂ATCGC₃TCT₂CTATA₃T₃G₃
NleH2 BamHI-f-His	G₄ATC₂ATGT₂ATCGC₃TCT₂CTATA₃T₃G₃
NleH2 KpnI-r-FLAG	C₂G₂TAC₂TATCT₂ACT₂A₂TACTACACTA₂TA₂GATC₂AGCT₃C₂
NleH2 XhoI-r-His	C₃TCGAGTATCT₂ACT₂A₂TACTACACTA₂TA₂GATC₂AGCT₃C₂
NleH2 XmaI-r	C₅G₃TATCT₂ACT₂A₂TACTACACTA₂TA₂GATC₂AGCT₃C₂
NleH1 XhoI-f	CTCGAGATGT₂ATCGC₂ATAT₂CTG
NleH1 NdeI-f-TEM	GCCATATGT₂ATCGC₂ATAT₂CTGTA₃T₃G₃
NleH1 KpnI-r-TEM	GCG₂TAC₂A₂T₄ACT₂A₂TAC₂ACACTA₂TA₂G
NleH2 NdeI-f-TEM	GC₂ATATGT₂ATCGC₃TCT₂CTATA₃T₃G₂
NleH2 KpnI-r-TEM	GCG₂TAC₂TATCT₂ACT₂A₂TACTACACTA₂TA₂G
NleH1 K159A-f	GCA₂CA₄GTC₂TG₂CGATGT₃ACTACATCTC
NleH1 K159A-r	GAGATGTAGTA₃CATCGC₂AG₂ACT₄GT₂GC
NleH2 K169A–f	CA₂CA₄GTGT₂G₂CGATGT₃ACTATATCTC
NleH2 K169A–r	GAGATATAGTA₃CATCGC₂A₂CACT₄GT₂G
RPS3 XhoI-f-HA	GC₂TCGAGATG₂CAGTGCA₃TATC₂A₂GA₂GAG₂
RPS3 NotI-r-HA	GCGCG₂C₂GCT₂ATGCTGTG₄ACTG₂CTG₄C
NleH1 NotI-r-HA	C₂GCG₂C₂GCT₂A₃T₄ACT₂A₂TAC₂ACACTA₂TA₂G
NleH2 NotI-r-HA	C₂GCG₂C₂GCT₂ATATCT₂ACT₂A₂TACTACAC
NleH1 pKD4-f	TGTATGT₂ATCGC₂ATAT₂CTGTA₃T₃G₃ATGT₂CTGTGTAG₂CTG₂AGCTGCT₂CG
NleH1 pKD4-r	GATA₄T₂ACTA₃T₄ACT₂A₂TAC₂ACACTA₂TA₂GCATATGA₂TATC₂TC₂T₂AG
NleH2 pKD4-f	C₂TCT₂CTATA₃T₃G₃ATGT₂CATG₂A₂T₂CT₃A₂CGTGTAG₂CTG₂AGCTGCT₂C
NleH2 pKD4-r	A₂TACTACACTA₂TA₂GATC₂AGCT₃C₂TC₂GTGATA₂GCATATGA₂TATC₂TC₂T₂A
NleH1 N-term-NotI-r-HA	GCGCG₂C₂GCT₂A₂TAGTC₂ACACTCG₂TA₂CTCTG₂CG
NleH1 C-term-XhoI-f-HA	GC₂TCGAGATGA₂CAG₂T₂GTCAGTAC₂TG₂TA₂TG
NleH2 N-term-NotI-r-HA	GCGCG₂C₂GCT₂AGT₂GCTA₂C₃TCG₂CA₂CTCTGAT₂G
NleH2 C-term-XhoI-f-HA	GC₂TCGAGATGA₂TA₃TCAC₃GTGC₂AG₂TA₂TG
NleH1 Y5S-f	ATGT₂ATCGC₂ATCT₂CTGTA₃T₃G₃ATGT₂C
NleH1 Y5S-r	GA₂CATC₃A₃T₃ACAGA₂GATG₂CGATA₂CAT
NleH1 N40S-f	GC₂GT₂CAT₂CTGATAGCG₃ACGCA₂GTA₂CG₂T₂G₂C
NleH1 N40S-r	GC₂A₂C₂GT₂ACT₂GCGTC₃GCTATCAGA₂TGA₂CG₂C
NleH1 A100T-f	CAC₂AGTGAGCACGACTATAGA₃GAG₂CTCTGT₂CAT₂CGA₂C
NleH1 A100T-f	GT₂CGA₂TGA₂CAGAGC₂TCT₃CTATAGTCGTGCTCACTG₂TG
GAPDH RT-f	AC₂AG₂TG₂TCTC₂TCTGACT₂C
GAPDH RT-r	GTG₂TCGT₂GAG₂GCA₂TG
IL-8 RT-f	CTG₂C₂GTG₂CTCTCT₂G
IL-8 RT-r	C₂T₂G₂CA₄CTGCAC₂T₂
NFKBIA RT-f	C₅TACAC₂T₂GC₂TGTG
NFKBIA RT-r	TCAGCAC₃A₂G₂ACAC₂A₂
TNFIAP3 RT-f	T₂GC₂TCATGCATGC₂ACT₂
TNFIAP3 RT-r	AGCA₃GC₄GT₃CA₂CA
PLK1 RT-f	GAG₂AG₂A₃GC₃TGACTGA
PLK1 RT-r	GCAGC₂A₂GCACA₂T₃GC
CENPE RT-f	GC₂A₂GACG₂AC₂A₂GCA
CENPE RT-r	TGCT₂GTAGCTGCGCT₂C₂T
IRF4 RT-f	TG₂T₂GC₂AG₂TGACAG₂A₂
IRF4 RT-r	TC₂AG₂T₂GCTG₂CGTCATA