Figure 5.
Expression of S-opsin and M-opsin in COUP-TFI-null and retinal-specific COUP-TFII-null mouse retina. A, B, S-opsin (A) and M-opsin (B) expression was examined by immunostaining using frozen-sectioned eyes from COUP-TFI knock-out mice (COUPI KO) at P23 and COUP-TFII retina-specific knock-out mice (COUP-TFIIfx/fx;Rx–-Cre; COUPII CKO) at 3 months of age. The signals were visualized using a secondary antibody conjugated to Alexa-488 (green). The nuclei were visualized by DAPI staining (blue). The middle and bottom are enlarged views of the dorsal and ventral periphery. The control samples were taken from each littermate of the COUP-TFI KO and COUP-TFII CKO mice. D, Dorsal; V, ventral.