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. 2009 Jun 14;59(1):12–20. doi: 10.1136/gut.2009.178020

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© Vicario et al 2010

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Analysis of the expression of mature IgE mRNA in oesophageal biopsies of EO and CTL patients. Patients are divided into atopic (EO-A, n = 7; CTL-A, n = 5) and non-atopic (EO-NA, n = 4; CTL-NA, n = 3) groups. The 412 bp IgE band was PCR amplified from oesophageal biopsies of EO and CTL patients, and GAPDH was amplified to control cDNA loading. Lanes − and + correspond to PCR negative and positive controls, respectively. A 1 kb plus DNA ladder was loaded. Gels are representative of one of three independent PCR amplifications yielding similar results. CTL, control; EO, eosinophilic oesophagitis; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PCR, polymerase chain reaction.