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NF-κB inhibition by MSA supplementation. Osteoblasts were cultured in Se-deficient medium (0.0182 μM) for 7 days before adding MSA to the culture system for another 7 days. On day 14, osteoblasts were treated with BCCM for 1 h and nuclear extracts were prepared. NF-κB activation was measured by (A) p65 translocation and (B) NF-κB gel mobility shift analysis (EMSA). Actin was used as a loading control; CC, cold oligonucleotide competitor; NS, non-specific binding.
