Fig. 5.

Osteoblasts response to BCCM following a short exposure to MSA. Osteoblasts were cultured in Se-deficient medium for 7 days before the addition of 4 μM MSA to the culture system for another 7 days. On day14, osteoblasts were treated for 2 h with VM or 50% BCCM in the presence or absence of 4 μM MSA. The supernatants were collected for enzyme-linked immunosorbent assay for (A) IL-6 and (B) MCP-1. Shown are the average values for 2 cultures carried out for each condition as indicated. (C) Some cells were treated for 1 h and the nuclear extracts were collected to assay translocation of p65 by western blotting. β-Actin was used as a loading control.