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. Author manuscript; available in PMC: 2009 Dec 10.
Published in final edited form as: Cell Cycle. 2008 Jul 1;7(13):2003–2012. doi: 10.4161/cc.7.13.6128

Fig. 3. The C-terminal half of ECT2 preferentially interacts with p190.

Fig. 3

(A) Co-immunoprecipitation of endogenous p190 with ECT2 variants. HeLa cells were transfected with wild type FLAG-ECT2, FLAG-ECT2N, or FLAG-ECT2C and synchronized at mitosis (M) or left as a cycling (C) population. One mg protein lysate was immunoprecipitated with mouse anti-p190 antibody (UBI). Immunoprecipitates were immunoblotted with mouse anti-p190 antibody (Becton-Dickinson) or anti-FLAG antibody. FLAG-ECT2, FLAG-ECT2N, and FLAG-ECT2C co-immunoprecipitated with p190 from both cycling and mitotic cell extracts, with preferential association observed between p190 and ECT2C.

(B) Western blotting of endogenous p190 or FLAG-tagged, wild type ECT2, ECT2N, and ECT2C overexpressing cells. Thirty µg whole cell lysates from cells in Panel A were immunoblotted with anti-FLAG antibody to detect levels of ECT2 or with anti-p190 (Becton Dickinson) to detect p190.