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. Author manuscript; available in PMC: 2009 Dec 10.
Published in final edited form as: Cell Cycle. 2008 Jul 1;7(13):2003–2012. doi: 10.4161/cc.7.13.6128

Fig. 4. Colocalization of endogenous p190 and ECT2 during mitosis.

Fig. 4

Hela cells were synchronized in mitosis, released for various lengths of time, and collected at each mitotic phase: inter- (interphase), pro- (prophase), meta- (metaphase), ana-(anaphase), telo- (telophase), and cytokinesis. Endogenous p190 and ECT2 were immunostained with mouse anti-p190 and rabbit-ECT2 antibody, respectively, and analyzed by immunofluorescence microscopy as described in Methods. Co-localization of p190 and ECT2 was observed in the cleavage furrow.