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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Dermatol Clin. 2010 Jan;28(1):93–105. doi: 10.1016/j.det.2009.10.011

Figure 4.

Figure 4

Demonstration of type VII collagen gene expression in epidermal keratinocytes (lane a), Ras oncogene transformed human epidermal keratinocytes (RHK) (lane b), human papilloma virus-transformed epidermal keratinocytes (HPK) (lane c), and skin fibroblasts (lane d). Total mRNA was isolated from the cultured cells and subjected to Northern hybridization with a human type VII collagen cDNA probe (middle panel). Note the expression of human type VII collagen mRNA of approximately 8.9 kb in epidermal keratinocytes and dermal fibroblasts (a, d). For reference, hybridizations were performed with the 230-kDa bullous pemphigoid antigen (BPAG1) and type I collagen (α1I) cDNAs. Rehybridization of the same filter got the GAPDH “housekeeping” gene served as an internal control for RNA loading. (Adapted from Ryynänen J, Sollberg S, Parente MG, et al. Type VII collagen gene expression by cultured human cells and in fetal skin. Abundant mRNA and protein levels in epidermal keratinocytes. J Clin Invest 1992; 89: 163–8., with permission).