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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Dermatol Clin. 2010 Jan;28(1):93–105. doi: 10.1016/j.det.2009.10.011

Figure 5.

Figure 5

Demonstration of the binding of type VII collagen to type IV collagen, and schematic representation of the dermal-epidermal junction with type VII collagen binding to basement membrane macromolecules. (A) Kinetic biosensor analysis demonstrates that recombinant NC-1 domain of human type VII collagen binds to type IV collagen (C-IV). After a wash, the NC-1/C-IV complex dissociates indicating reversible nature of the binding. Addition of laminin-332 (laminin-5, L-5) did not result in additional binding, suggesting that the L-5 binding site corresponds to or is located close to that for binding of type IV collagen. (B) Binding of the NC-1-VII collagen domains to C-IV, L-5, and laminin-6 (L-6). (C) The anchoring fibrils entrap type VII collagen fibers, stabilizing the association of lamina densa of the dermo-epidermal basement membrane to underlying dermis. (Adapted from Brittingham R, Uitto J, Fertala A. High-affinity binding of the NC-1 domain of collagen VII to laminin 5 and collagen IV. Biochem Biophys Res Commun 2006; 343: 692–9., with permission).