Figure 6. Dh404-induced activation of Nrf2 in H9C2 cardiomyocyte.

Sub-confluent cells were transfected with ARE-luc (200 ng) and pRL-TK (10 ng) for 24 hours, and then cultured with serum-free DMEM for another 24 hours. The quiescent cells were stimulated with dh404 as indicated, and then subjected to ARE reporter assays (A) as well as immunoblot analysis of cytosolic and nuclear Nrf2 expression (B, left panel) or immunochemical staining of Nrf2 (B, right panel).