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. 1998 Sep 1;95(18):10453–10458. doi: 10.1073/pnas.95.18.10453

Figure 2.

Figure 2

The hydrophobic patch on cyclin A recruits RXL proteins. (A) Binding to p107 was assayed by immunoprecipitation and Western blotting of p107 or HA-tagged cyclin A in lysates from transfected U2OS cells. (B) Binding to E2F1 was assayed by immunoprecipitation and Western blotting of E2F1 or HA-tagged cyclin A. Lysates from U2OS cells transfected with either cyclin A- or cyAhpm-cdk2 were mixed with recombinant E2F1 before immunoprecipitation. (C) Binding to p21 was detected by Western blotting for Myc-tagged cyclin A or cdk2 after immunoprecipitation of p21 from transfected U2OS cells. (D) Binding to p27 was assayed by glutathione-agarose precipitation of GST or GST-p27 and Western blotting for Myc-tagged cyclin A or cdk2. Lysates from U2OS cells transfected with either cyclin A or cyAhpm and/or cdk2 were mixed with recombinant GST or GST-p27 before precipitation.