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The hydrophobic patch on cyclin A is important for phosphorylation of a subset of substrates. HA-tagged cyclin A–cdk2 or cyAhpm–cdk2 complexes immunoprecipitated from transfected U2OS cells were incubated with [γ-32P]ATP and the following amounts of the following substrates: histone H1, 100 ng, 250 ng, 1 μg, and 2 μg; p107, 10 ng, 50 ng, 200 ng, and 500 ng; E2F1, 10 ng, 50 ng, 200 ng, and 500 ng; Rb-C, 10 ng, 50 ng, 200 ng, and 1 μg. Autophosphorylated cyclin A is indicated with ∗.
