Fig. 2.

Parametric analyses. (A) Ethanol preexposure-induced loss of olfactory startle increases with both ethanol concentration and length of a single exposure. Flies were exposed to increasing E/A of 50:100, 70:80, or 100:50 for 0, 20, 40, or 60 min, respectively, and the degree of startle in response to ethanol was measured 2 days later. (*, P < 0.01, **, P < 0.001). (B) The degree of morphological damage of antennae also increased with exposure number. Flies were given a single 100:50 E/A exposure once, or once per day, for 4 consecutive days and analyzed 2 days later (*, P < 0.01 for all comparisons). (C) Olfactory damage is permanent. Flies were assayed 2, 7, and 9 days after a single exposure to ethanol. Recovery was never observed.