Fig. 7.

Localization of trypsin in eel sperm. (A) Immunohistochemical analysis of whole cells using an anti-eel trypsinogen antibody. Trypsin localizes at the sperm membrane and within the mitochondria in the caput end of the sperm head. (Scale bar, 10 μm.) (B) Gelatin zymography assay and Western blot analysis of the eel sperm membrane with an anti-trypsinogen antibody (Anti-TN). PMSF − and + indicate with or without Phenylmethylsulfonyl fluoride, respectively. Arrowheads indicate molecular markers (Left), trypsin activity assayed by zymography, or signal detection by Western blot analysis using the anti-eel trypsinogen antibody.