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A simplified scheme showing the reactions of standard [NiFe]-hydrogenases. Active hydrogenase molecules catalyze H2/H+cycling, and are subject to aerobic inactivation, forming either the Ready or Unready forms, depending on the availability of electrons and protons. The Ready form can also be formed anaerobically at oxidizing potentials. (Adapted from ref. 20 with permission from the Royal Society of Chemistry.)
