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. Author manuscript; available in PMC: 2010 Nov 19.
Published in final edited form as: Cell Host Microbe. 2009 Nov 19;6(5):433–445. doi: 10.1016/j.chom.2009.09.013

Figure 6. CPXV012 inhibits peptide loading of MHC-I.

Figure 6

(A) and (B) H2-Kb thermostability in the presence of virus expressed CPXV012. MC57 cells were infected with indicated viruses. At 6 hpi, cells were pulse-labeled for 20 min followed by 2 h chase. Lysates were incubated at indicated temperatures in the presence or absence of the peptide SIINFEKEL. H2-Kb was immunoprecipitated with mab Y3 and EndoH-treated. (C). Thermostability of HLA-A3 transfected in HeLa together with CPXV012-N-FLAG or CPXV012-C-FLAG. Cells were labeled as in A and incubated at indicated temperature in the presence or absence of peptide ILRGSVAHK. “R” and “D” refer to EndoH-resistant and digested MHC-I.