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. 2010 Jan 8;6(1):e1000801. doi: 10.1371/journal.pgen.1000801

Figure 2. WRN-1 is required for the phosphorylation of CHK-1(S345) induced by inhibition of DNA replication.

Figure 2

Wild-type N2, wrn-1(gk99), atl-1(RNAi), atm-1(gk186), and chk-1(RNAi) worms were treated with 25 mM hydroxyurea (HU) from the L4 stage for 16 h. To knockdown atl-1 (ATR homolog) or chk-1 expression, worms were fed E. coli cells expressing the cognate double-stranded RNA from the L1 stage to the adult stage. (A) Phosphorylation of CHK-1(S345) in premeiotic germ cells probed with phospho-CHK1(S345) antibody. Magnification bar, 10 μm. (B) Worm extracts analyzed by western blotting using antibodies to phospho-CHK1(S345), and to α-tubulin as a control.