Skip to main content
. 2010 Jan 8;6(1):e1000801. doi: 10.1371/journal.pgen.1000801

Figure 3. The wrn-1 mutation does not affect the phosphorylation of CHK-1(S345) after UV-irradiation.

Figure 3

Wild-type N2, wrn-1(gk99), atl-1(RNAi), atm-1(gk186), and chk-1(RNAi) worms were irradiated as one-day-old adults with UV radiation (100 J/m2) and cultured for 1 h before immunostaining. atl-1 and chk-1 expression was knocked down as in Figure 2. Phosphorylation of CHK-1(S345) was probed as in Figure 2 by (A) immunostaining of mitotic germ cells and (B) western analysis of worm extracts. Magnification bar, 25 μm.