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. 2009 Dec 24;5(12):e1000705. doi: 10.1371/journal.ppat.1000705

Figure 5. Interaction between p33 replication protein and Vps23p ESCRT-I and Bro1p accessory ESCRT proteins.

Figure 5

The split ubiquitin assay was used to test binding between p33 and (A) Vps23p; (B) Bro1p, or (C) the N-terminal UEV domain of the yeast Sc-Vps23p or two Arabidopsis and two Nicotiana homologs in wt (NMY51) or vps4Δ yeast. The bait p33 was co-expressed with the shown prey proteins. SSA1 (HSP70 chaperone), and the empty prey vector (NubG) were used as positive and negative controls, respectively.