Figure 6.

c-maf Is Expressed in IL-10-Producing Th1, Th2, and Th17 Cells

CD4⁺ T cells from DO11.10 TCR transgenic animals were differentiated for 1 week in medium containing increasing doses of OVA plus DC in the absence (Med) or presence of IL-12, as indicated. On day 7, cells were (A) restimulated for cytokine expression or (B) not restimulated for transcription factor expression as in Figure 1. CD4⁺ T cells from C57Bl/6 animals were isolated and cultured for 5 days in the presence of plate-bound anti-CD3 (50 ng/ml), soluble anti-CD28 (50 ng/ml), IL-6 (50 ng/ml), TGF-β (1 ng/ml), and IL-1 (10 ng/ml); Th17 cells were restimulated on day 5 (for cytokine expression) or not (for transcription factor expression) as in Figure 5C. Th1 and Th17 cells were cultured as in Figure, all in the absence (−; Control), with DMSO, or presence (+) of the PD184352 MEK inhibitor. Transcription factor expression is shown in (D). Similar results were obtained from three (A, B, and C) or two experiments (D, with two doses of inhibitor or control). Cytokine and transcription factor expression were analyzed from RNA isolated from all populations by RT real-time PCR.