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Anticipated fragments produced by cleavage of the fully exchanged product of transfer of the complementary strand of linear pBluescript SK(+) dsDNA to 32P-labeled circular ssDNA with pairs of restriction endonucleases. The following pairs of restriction endonucleases were used to generate the fragments shown: BamHI (Bam) and AflIII (Af) (434 bp), BamHI and AlwNI (Al) (846 bp), KpnI (Kpn) and BsaAI (Ba) (425 bp), or KpnI and BsaHI (Bh) (1,029 bp).
