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. 1998 Sep 1;95(18):10477–10481. doi: 10.1073/pnas.95.18.10477

Figure 3.

Figure 3

Kinetics and nature of heteroduplex DNA formation when strand exchange is initiated at either a 3′ (A) or 5′ (B) overhanging end of the linear dsDNA’s complementary strand. 32P-labeled pBluescript SK(+) circular ssDNA was incubated with Rad51 and RPA, and then the reaction was initiated by the addition of linear dsDNA with either 3′ or 5′ overhanging ends. After 20 min and 4 hr, the extent of heteroduplex DNA formed was determined from the amount of 32P-label in the specific dsDNA fragments produced by cleavage with the restriction endonucleases indicated in Fig. 2. The ordinate represents the mol percent of circular ssDNA that has been incorporated into each of the fragments, and the abscissa represents the linear “map” of the DNA.