Figure 4. Kinetic basis for Cdc34-SCF processivity.

The millisecond kinetics of a single encounter reaction were fit to a sequential model revealing estimates for individual transfer and dissociation rates for each intermediate in the generation of polyubiquitylated CycE (blue numbers) and β-Cat (red numbers) products. The percentages listed above or below each product were the percentages from the final product distributions (λ) shown in Fig. 1b and Fig. 3b.