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APC co-factor activity of murine plasma protein S. The FVa-based assay is described in the Design and Methods sections. To vary plasma protein S levels, pooled normal mouse plasma as a source of protein S was mixed in various proportions with rat PSdP. 0% mouse plasma PS contains 100% rat PSdP. Clotting time was prolonged in a linear manner with increasing doses of normal mouse plasma containing protein S.
