Figure 3.

Immune modulatory effect of UCB-MSC on the expansion of T and NK-lymphocyte subsets, induced by allogeneic stimulus. Recovery of total number of lymphocytes (A), CD3⁺ (B), CD3⁺CD4⁺ (C), CD3⁺CD8⁺ (D), CD3^{neg s}CD56⁺ NK cells (E), CD4⁺CD25⁺ (F), CD4⁺CD25^bright ( G ) T-lymphocytes subsets and with respect to the initial number (white columns), was assessed after 10 days of primary culture (gray columns). Percentages of CTLA4⁺ and Foxp3⁺ cells were calculated on gated CD4⁺CD25⁺ T cells ( H ). MLC was performed in the absence (Ctrl-MLC) or presence of third-party MSC derived from UCB3 (MLC+UCB3-MSC) or UCB6 (MLC+UCB6-MSC). Results are expressed as the number of cells/mL in panels A–E and as percent of positive cells in panels F–H.