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. 2009 Jul 6;86(4):891–902. doi: 10.1189/jlb.0209050

Figure 2.

Figure 2.

LL-37 exposure of PMN in the presence of modifiers of apoptotic pathways. Freshly isolated human PMN were incubated with LL-37 (at the concentrations indicated) for 20 h in the presence or absence of 20 ng/ml GM-CSF (A), for 6 h in the presence or absence of 20 μM R-Roscovitine (B), or for 20 h in the presence or absence of 500 ng/ml E. coli LPS (C and D). Cell death was examined by FACS analyses. Figures represent mean values ± sem for n ≥ 3 donors performed in triplicate. Significance was assessed by one-way ANOVA with Bonferroni’s multiple comparison test comparing each treatment with control; *, P ≤ 0.05; **, P ≤ 0.01, for necrotic (A, B, and D) or viable (C) cells in LL-37-treated samples compared with controls without LL-37 in the presence of (A) GM-CSF, (B) R-Roscovitine, or (C) LPS; #, P < 0.001, for necrotic (D) or viable (C) cells in LPS-treated samples compared with controls without LPS in the comparable concentration of LL-37.