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Northern blot analysis of lysyl hydroxylase 3 mRNA in human tissues and cells. Each lane in A–C contains 2 μg of poly(A)+ RNA from the adult (A and B) or fetal (C) tissue indicated. Each lane in D contains 15 μg of total RNA from adult human Saos-2 osteosarcoma cells or skin fibroblasts. Blots were hybridized with a 354-bp PCR fragment from the 3′ end of the cDNA. Autoradiography time was 4 (A–C) or 7 (D) days.
