Fig. 4.

Tregs suppress the myelopoietic activity of hematopoietic cytokine-producing effector T cells. CD4⁺ CD25^- T cells were cultured together with increasing numbers of Tregs (i.e. 0×, 0.25×, 0.5×, 1.0× and 2.0× of the number of CD4⁺CD25^- T cells) for 5 days, and the conditioned media were examined for their activity in production of GM-CSF and IL-3 (A) and supporting the colony formation of splenic myeloid progenitors (B). The production of cytokines was examined by ELISA and intracellular staining (A). Error bars are differences of duplicated measurements. Representative data of 3 independent experiments are shown.