Table 1.

List of Monoclonal Antibodies and Their Target Antigens Used for Immunostaining and Immunofluorescence

Antibody	Supplier	Conditions	Target antigen
UEA-1 lectin	Vector Laboratories (Burlingame, CA)	Heat induced temperature retrieval, 1:300 dilution for 1 h	Human EC (does not stain rat EC)
ED1 (MCA341) and ED2 (MCA342)	AbD Serotec (Raleigh, NC)	1% pepsin pretreatment, 1:600 for 1 h	Rat macrophages and monocytes (does not stain human cells)
vWf (CL20176A-R)	Cedarlane (Burlington, Canada)	After UEA-1 application, 1:5000 overnight	Rat EC and platelets (does not stain human cells)
SMA (AB5694)	Abcam (Cambridge, MA)	After UEA-1 application, 1:1000 overnight	Rat smooth muscle cells (does not stain rat EC)
TUNEL assay (DNA polymerase 1 large [Klenow] fragment, dATP, dCTP, dGTP, and Bio-11-dUTP)	Promega (Nepean, Canada)	1% pepsin pretreatment, cocktail applied for 1 h at 37°C	Apoptotic cells (stains fragmented DNA of both rat and human cells)

UEA-1, Ulex Europaeus Agglutinin I; vWF, von Willebrand factor; SMA, smooth muscle α-actin.