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Thickness of constructs for the culture medium formulations described in Table 1, measured through differential interference contrast (DIC) microscopy. Results are average ± standard error of a minimum of four and a maximum of six independent experiments. *Significantly different to all other groups and **significantly different to serum-free groups. (a) 3 days, (b) 1 week, (c) 2 weeks, and (d) 3 weeks.
