FIG. 4.

Fluorescent images of 4-week cell sheets cultivated in 1% FBS + 1% ITS (a–c), 1% FBS (d–f), or 10% FBS (g–i) and stained with a phycoerythrin-conjugated antibody for α-smooth muscle actin (a, d, g), a collagen type I polyclonal antibody and a fluorescein isothiocyanate–conjugated secondary antibody (b, e, h), or phalloidin-rhodamine (c, f, i). All images were acquired using a 20 × objective; however, some images were cropped to remove artifacts. Scale bars are 20 μm. Asterisks in bindicate cell processes or filipodia.