FIG. 9.

Images from 3-week bovine corneal stroma fibroblasts cultivated in the culture medium supplemented with 1% FBS + 1% ITS: (a–d) cross-sectional traditional TEM and (e) quick-freeze/deep-etch (QFDE)/TEM. Arrowheads point to cell–cell junctions. Note the presence of matrix fibrils in between contacting cells. M, mitochondria; G, Golgi apparatus; E, rough endoplasmic reticulum; R, residual bodies; N, cell nucleus. (d, e) Close-up details of intracellular residual bodies with a concentric-ring shape observed in a traditional cross section. Scale bars are 500 nm in (a–c) and 100 nm in (d, e).