Table 1.

Antithrombotic and Prothrombotic Gene Expression in Umbilical Cord Blood–Derived Endothelial Progenitor Cells and Human Aortic Endothelial Cells

Surface	FN				SMC
Flow conditions	Static		Flow		Static		Flow
Gene	CB-EPC	HAEC	CB-EPC	HAEC	CB-EPC	HAEC	CB-EPC	HAEC
eNOS	1.0 ± 0.2	1.4 ± 0.4	6.0 ± 0.8a	4.9 ± 1.1a	1.5 ± 0.2	1.1 ± 0.2	5.9 ± 0.9a	3.8 ± 0.6a
COX2	1.2 ± 0.2	0.9 ± 0.3	6.7 ± 0.9a,b,c	2.2 ± 0.2	0.8 ± 0.2	0.5 ± 0.1	12.5 ± 0.7a,b	8.9 ± 0.2a,c
KLF-2	1.5 ± 0.6	0.6 ± 0.3	9.1 ± 1.2a	5.2 ± 1.8d	1.9 ± 0.5e	0.6 ± 0.3	7.3 ± 1.8a	4.0 ± 0.4a
Thrombomodulin	2 ± 0.1	2.1 ± 0.4	10.6 ± 1.9a	10.7 ± 1.9d	2.6 ± 0.4	5.8 ± 0.3	8.5 ± 1.6d	10.6 ± 1.7d
tPA	0.7 ± 0.1c	0.5 ± 0.1c	0.5 ± 0.1c	1.4 ± 0.1c	1.3 ± 0.04	1.1 ± 0.1	0.9 ± 0.08	0.9 ± 0.1
ET-1	1.0 ± 0.1c,e	0.4 ± 0.1	0.5 ± 0.1	0.3 ± 0.1	0.4 ± 0.04	0.3 ± 0.1	0.5 ± 0.1	0.4 ± 0.1
Cyclin D	0.9 ± 0.1	0.8 ± 0.1	0.8 ± 0.2	0.5 ± 0.1d	0.7 ± 0.1	0.9 ± 0.04	0.7 ± 0.1	0.6 ± 0.04a
VCAM-1	0.4 ± 0.1	0.2 ± 0.03	2.0 ± 0.8	0.9 ± 0.2d	0.2 ± 0.1	0.1 ± 0.02	1.1 ± 0.6	2.1 ± 0.5a
ICAM-1	0.5 ± 0.1	0.7 ± 0.1	4.8 ± 1.3a	0.5 ± 0.1c	0.2 ± 0.1	0.2 ± 0.1	4.3 ± 2.2a	5.9 ± 0.9a
E-selectin	0.9 ± 0.2	1.7 ± 1.1	5.2 ± 3.4	1.7 ± 0.8c	0.5 ± 0.3	0.2 ± 0.1b	12.2 ± 11	90.7 ± 9.2a

Comparison of antithrombotic and prothrombotic genes in CB-EPCs and HAECs on either FN or SMC surfaces exposed to static or flow conditions. The underlined values represent differences in the two cell types, and the boldfaced values are the significant findings.

^ap < 0.01 and ^dp < 0.05, compared to equivalent static conditions.

^bp < 0.01 and ^ep < 0.05, compared to equivalent HAEC conditions.

^cp < 0.05, compared to equivalent SMC conditions.

CB-EPCs, umbilical cord blood–derived endothelial progenitor cells; HAEC, human aortic endothelial cells; FN, fibronectin; SMC, smooth muscle cell; eNOS, endothelial nitric oxide synthase; COX2, cyclooxygenase-2; ET-1, endothelin-1; tPA, tissue plasminogen activator; KLF-2, Kruppel-like factor-2; VCAM-1, vascular cell adhesion molecule 1; ICAM-1, intracellular adhesion molecule 1.