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Expression of cartilage markers in MSC-laden constructs with variation of initial seeding density. Relative gene expression normalized to 3-week control constructs (dashed line) of (A) M20 and (B) M60 constructs at 7 weeks in + and T media. The chondrocyte-like phenotype is maintained in constructs cultured in T medium 4 weeks after TGF-β3 withdrawal. Data from two experiments are presented.
