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. 2009 Nov 4;15(12):3923–3930. doi: 10.1089/ten.tea.2008.0653

FIG. 3.

FIG. 3.

Angiogenesis in microchannels of in vivo harvested synovial joint condyle graft. Gross images of distal ends of in vivo retrieved condyle grafts revealed apparent tissue ingrowth into the scaffolds' microchannels seeded without human cells (A), hMSCs (B) and hMSC-derived osteogenic cells (hMSC-Ob) (C). Hematoxylin and eosin–stained microscopic sections showed pronounced vascularization in a MSC-seeded graft (E, H) in comparison with modest vascularization in grafts seeded with MSC-derived osteoblasts (F, I) or without delivered cells (cell-free) (D, G), which nonetheless was populated by host-derived cells. Quantitatively, grafts seeded with MSCs showed not only significantly more blood vessels, but also vessels with significantly greater diameter than MSC-Ob or cell-free samples (J) (n = 10; p < 0.05). There was no significant difference in vascularization between MSC-Ob and cell-free samples (n = 10; p > 0.05) (G). Scale of D, E, F: 400 μm, G, H, I: 200 μm. *, p > 0.05. Color images available online at www.liebertonline.com/ten.