Table 1.
Experimental Design Indicating the Number of Sections for the Characterization of the Human Umbilical Vein (HUV) Specimens, the Decellularized Constructs, and Their Repopulation by Vocal Fold Fibroblasts at Different Time Points
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Fibroblast-repopulated scaffolds |
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|---|---|---|---|---|---|---|---|---|---|---|---|---|
| |
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Days in culture |
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| Experimental characterization | Native umbilical cord specimens | Acellular HUV constructs | 0 | 1 | 3 | 6 | 9 | 12 | 15 | 18 | 21 | |
| SEM | 3 | 3 | ||||||||||
| Light microscopy with H&E and Alcian blue | 3 | 3 | 3 | |||||||||
| Light microscopy with trichrome (for image analysis) | 3 | 3 | 3 | |||||||||
| Cell recovery (cell proliferation) | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | |||
| Rheometric testing | 6 | 3 | 3 |