FIG. 12.

Sections of middle phalanx models stained with von Kossa solution to show phosphate as an indicator of mineral formation when correlated with Alizarin red staining for calcium. Constructs were implanted for 20 weeks and have midshafts of P(LA-CL), HA-P(LA-CL), or β-TCP-P(LA-CL). Patterns of phosphate are different for each type of midshaft, and enlargements of specific regions (boxed outlines) illustrate varying staining distributions (black deposits indicated by arrows, for example, in the lower middle panel). Magnification = 1.8× (upper panels) and 70 × (lower panels). Color images available online at www.liebertonline.com/ten.