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. Author manuscript; available in PMC: 2010 Aug 1.
Published in final edited form as: Biomaterials. 2009 Apr 26;30(23-24):3926–3933. doi: 10.1016/j.biomaterials.2009.03.056

Figure 4. Cellular uptake of SPIO in isolated Kupffer cells.

Figure 4

Non-labeled SPIO were preincubated with plasma or PBS and added to a suspension of isolated Kupffer cells in PBS/BSA. A, The effect of plasma on nanoparticle uptake as determined by microscopy. The particles were detected with a FITC-labeled anti-dextran antibody (green). Macrophages were stained with F4/80 antibody (red). The cells were examined by fluorescent microscopy. Objective, ×60. Upper panel, PBS-treated particles: lower panel, plasma-treated particles. B, Quantification of SPIO uptake by mouse Kupffer cells in the presence or absence of mouse plasma. As an additional control, mouse plasma depleted for SPIO-binding proteins was used (right bar). Average of 2 experiments are shown.