FIG. 6.

HepG2 hepatocytes cultured on electrospun biodegradable scaffolds. (A) Scanning electron microscopy (SEM) images of polylactic acid (PLA) fibers. (B) Average fiber diameter was determined from image analysis of SEM micrographs. (C) Brightfield (4×) and fluorescent image of PLA scaffold cultured with HepG2 hepatocytes pre-stained using CellTracker Orange CMTMR. Fluorescent image shows islands of HepG2 cells cultured into an 8 × 8 grid pattern. (D) SEM micrograph of an electrospun mesh of poly-lactic acid printed with extracellular matrix (ECM) and seeded with cells. Inset shows depression made on mesh after ECM printing using a manual arrayer. Scale bar of inset is 500 μm. Color images available online at www.liebertonline.com/ten.