Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2008 Oct 21;15(6):1247–1255. doi: 10.1089/ten.tea.2008.0249

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright 2009, Mary Ann Liebert, Inc.

PMC Copyright notice

FIG. 2. — (A) Collagens were extracted from 5- and 8-week monolayer cultures grown in medium with or without 100 ng/mL of bone morphogenetic protein (BMP)-2. Two μg of total protein extracted was resolved on a 7.5% Tris-hydrochloric acid gel and western blotted for type I and type II collagen. (B) Immunohistochemical analysis for types I and II collagen in 5- and 8-week culture tissue from control and BMP-2–stimulated monolayer cultures. Specific labeling was developed using enzyme substrate vector red substrate kit. Images were taken at 10× magnification.

FIG. 2. — (A) Collagens were extracted from 5- and 8-week monolayer cultures grown in medium with or without 100 ng/mL of bone morphogenetic protein (BMP)-2. Two μg of total protein extracted was resolved on a 7.5% Tris-hydrochloric acid gel and western blotted for type I and type II collagen. (B) Immunohistochemical analysis for types I and II collagen in 5- and 8-week culture tissue from control and BMP-2–stimulated monolayer cultures. Specific labeling was developed using enzyme substrate vector red substrate kit. Images were taken at 10× magnification.