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. 1998 Sep 1;95(18):10487–10492. doi: 10.1073/pnas.95.18.10487

Figure 3.

Figure 3

Detection of T2- and C2-specific sequences from the progeny T1T2C2C3 isolated after single lesion transfer. (A) Agarose gel electrophoresis of segment-specific RT-PCR products amplified from viral RNAs isolated after two passages of single lesion transfer in N. tabacum. Lanes 1–4 contain RT-PCR products from four independent isolates of T1T2C2C3 for detection of TAV RNA 2 (T2 PCR). Lanes 5–7 contain PCR products for CMV RNA 2 (C2 PCR) from the RNAs used for lanes 2–4, respectively. Lane C contains a positive control of a 793-bp T2-specific DNA fragment. (B) Reamplification of PCR products (RE-PCR) using the same primer pair as in A and each DNA (2 μl out of 100 μl) from the samples of lanes 1–4 in A. Lane C contains a positive control for T2 as used in A. Note that there is no amplification of T2-specific DNA even after reamplification of PCR products generated in A. Lane M is a 100-bp DNA ladder.