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Interactions between the V-TAV 1a protein and the CMV-Y 2a protein. Detection of positive blue colonies indicates in vivo protein–protein interactions. Note that the 1a protein from V-TAV(J) (wild-type, wt T1a) was not bound to the 2a protein from CMV-Y RNA 2 (C2a), whereas the 1a protein from T1C2(C2-T2)C3 was bound to C2a.
