. Author manuscript; available in PMC: 2009 Dec 11.

Published in final edited form as: ACS Chem Biol. 2009 Oct 16;4(10):844–854. doi: 10.1021/cb900167m

TABLE 2.

Thermodynamic effects of doxorubicin binding on HIV-1_posctrlRNA^a

Ratio of compound to RNA	T_m1 (°C)	T_m2 (°C)	Hyperchromicity increase (%)^b
0:1	36.1 ± 0.3	82.0 ± 0.3	21.3 ± 0.6
1:1	37.1 ± 0.3	82.1 ± 0.3	26.0 ± 0.7
5:1	39.3 ± 0.3	82.5 ± 0.3	26.4 ± 0.4
10:1	41.0 ± 0.3	83.0 ± 0.3	28.2 ± 1.0

Values listed represent the best-fit solutions to the van't Hoff equation. Fitting errors for T_m were less than the nominal error of the temperature probe (0.3 °C, Varian); therefore all T_m errors were set to 0.3 °C.

Percentage increase in UV absorbance observed at 260 nm from 20 to 95 °C.